Composite

Part:BBa_K2387017:Design

Designed by: Stijn Prinsen   Group: iGEM17_Wageningen_UR   (2017-10-16)


Cpx system reporter + CpxP-Affinity molecule inhibitor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1587


Design Notes

Strong tac promoter + strong RBS were chosen to increase CpxP-Aff production levels


Source

The mRFP1 under control of pCpxR originates from biobrick BBa_K339007.

The CpxP-Affinity molecule fusion originates from biobrick BBa_K2387015

References